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验证数据展示

  • WB analysis using 32474-1-AP

    WB analysis using 32474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32474-1-AP (ELOVL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ELOVL3 Polyclonal antibody

ELOVL3 Polyclonal Antibody for WB, ELISA
Cat No. 32474-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

3-keto acyl-CoA synthase ELOVL3, Cold-inducible glycoprotein of 30 kDa, EC:2.3.1.199, Elongation of very long chain fatty acids protein 3, ELOVL FA elongase 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse adipose tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32474-1-AP targets ELOVL3 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 ELOVL3 fusion protein Ag24318 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3
别名 3-keto acyl-CoA synthase ELOVL3, Cold-inducible glycoprotein of 30 kDa, EC:2.3.1.199, Elongation of very long chain fatty acids protein 3, ELOVL FA elongase 3
计算分子量 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC034344
基因名称 ELOVL3
Gene ID (NCBI) 83401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HB03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELOVL3 (elongation of very long-chain fatty acids 3) is a member of the ELOVL family of enzymes that synthesize very long chain fatty acids (VLCFAs). ELOVL3 is expressed primarily in brown and white adipose tissue, skin sebaceous glands, and the liver, synthesizes C20-C24 saturated and monounsaturated fatty acids (PMID: 37847682). ELOVL3 is involved in the regulation of the progression of adipogenesis through activation of peroxisome proliferator-activated receptor (PPAR)γ in mouse adipocytic 3T3-L1 cells (PMID: 22436697, 16326704).

实验方案

Product Specific Protocols
WB protocol for ELOVL3 antibody 32474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols