验证数据展示
经过测试的应用
| Positive WB detected in | human artery tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human lung tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15257-1-AP targets Elastin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Elastin fusion protein Ag7179 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elastin |
| 别名 | ELN, WS, WBS, Tropoelastin, SVAS |
| 计算分子量 | 68 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC065566 |
| 基因名称 | Elastin |
| Gene ID (NCBI) | 2006 |
| RRID | AB_2878120 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15502 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Elastic fibers are an abundant and integral part of many extracellular matrices, in which they provide the elastic properties to tissues such as arterial, lung, and skin. Elastic fibers are consisting of an elastin core surrounded by a mantle of fibrillin-rich microfibrils (PMID: 12082143). Elastin is an extremely durable, insoluble biopolymer formed through the lysine-mediated crosslinking of its soluble precursor tropoelastin, which is an approximately 60-70 kDa protein (PMID: 15837523). Deletions and mutations in the elastin gene (ELN) are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Elastin antibody 15257-1-AP | Download protocol |
| IHC protocol for Elastin antibody 15257-1-AP | Download protocol |
| IP protocol for Elastin antibody 15257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Cell Mol Biol Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation. | ||
J Med Chem Design, Synthesis, and Biological Evaluation of an Orally Bioavailable, Potent, and Selective ROCK2 Inhibitor for Psoriasis Treatment | ||
Int J Mol Sci αvβ3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis | ||
J Cell Physiol Novel insight into the role of clusterin on intraocular pressure regulation by modifying actin polymerization and extracellular matrix remodeling in the trabecular meshwork. | ||
Am J Physiol Cell Physiol SEMA3B inhibits TGFβ-induced extracellular matrix protein production and its reduced levels are associated with a decline in lung function in IPF | ||
Polymers (Basel) The Development of a 3D PET Fibrous Scaffold Modified with an Umbilical Cord dECM for Liver Tissue Engineering |
