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验证数据展示

  • WB analysis using 12727-1-AP (same clone as 12727-1-PBS)

    WB analysis using 12727-1-AP (same clone as 12727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12727-1-PBS in a different storage buffer formulation.

  • WB analysis using 12727-1-AP (same clone as 12727-1-PBS)

    WB analysis using 12727-1-AP (same clone as 12727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12727-1-AP (ELL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12727-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12727-1-AP (same clone as 12727-1-PBS)

    IP experiment of mouse brain using 12727-1-AP (same clone as 12727-1-PBS)

    IP result of anti-ELL2 (IP:12727-1-AP, 4ug; Detection:12727-1-AP 1:300) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 12727-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ELL2 Polyclonal antibody, PBS Only

ELL2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 12727-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

ELL 2

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

12727-1-PBS targets ELL2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3428

Product name: Recombinant human ELL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-640 aa of BC028412

Sequence: TNRVPPTLNGHLNPTSEKSAAGLPLPPAAAAIPTPPPLPSTYLPISHPPQIVNSNSNSPSTPEGRGTQDLPVDSFSQNDSIYEDQQDKYTSRTSLETLPPGSVLLKCPKPMEENHSMSHKKSKKKSKKHKEKDQIKKHDIETIEEKEEDLKREEEIAKLNNSSPNSSGGVKEDCTASMEPSAIELPDYLIKYIAIVSYEQRQNYKDDFNAEYDEYRALHARMETVARRFIKLDAQRKRLSPGSKEYQNVHEEVLQEYQKIKQSSPNYHEEKYRCEYLHNKLAHIKRLIGEFDQQQAESWS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor, RNA polymerase II, 2
别名 ELL 2
计算分子量 640 aa, 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC028412
基因名称 ELL2
Gene ID (NCBI) 22936
RRIDAB_10644131
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00472
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998).

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