验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse stomach tissue, rat brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12727-1-AP targets ELL2 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ELL2 fusion protein Ag3428 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elongation factor, RNA polymerase II, 2 |
| 别名 | |
| 计算分子量 | 640 aa, 72 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC028412 |
| 基因名称 | ELL2 |
| Gene ID (NCBI) | 22936 |
| RRID | AB_10644131 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00472 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELL2 (Elongation Factor for RNA Polymerase II 2) is a key transcriptional elongation factor that regulates RNA polymerase II (Pol II) during mRNA synthesis (PMID: 34265249). It belongs to the ELL gene family, which includes ELL1, ELL2, and ELL3, and plays a crucial role in controlling the elongation phase of transcription (PMID: 9108030). ELL2 is an androgen-responsive gene that is expressed by prostate epithelial cells and is frequently down-regulated in prostate cancer (PMID: 29179998).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELL2 antibody 12727-1-AP | Download protocol |
| IP protocol for ELL2 antibody 12727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.
| ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
J Biol Chem Cytoplasmic poly(A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells. | ||
FASEB J Androgen receptor splicing variant 7 (ARv7) promotes DNA damage response in prostate cancer cells | ||
Cell Death Dis TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response | ||
