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ELL Polyclonal antibody

ELL Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51044-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

C19orf17, ELL, ELL1, MEN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP, CoIP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ELL fusion protein Ag0617 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor RNA polymerase II
别名 C19orf17, ELL, ELL1, MEN
计算分子量 500 aa, 68 kDa
观测分子量 68-80 kDa
GenBank蛋白编号BC049195
基因名称 ELL
Gene ID (NCBI) 8178
RRIDAB_2099137
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55199
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

实验方案

Product Specific Protocols
WB protocol for ELL antibody 51044-1-APDownload protocol
IHC protocol for ELL antibody 51044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Nat Commun

Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.

Authors - Chia-Yu Chung
  • KD Validated
humanWB

Nat Commun

ELL targets c-Myc for proteasomal degradation and suppresses tumour growth.

Authors - Yu Chen
mouseWB

Oncogene

The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia.

Authors - Hao Wang
humanWB

Prostate

ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.

Authors - Liu Lingqi L
  • KD Validated
humanWB

Mol Immunol

Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.

Authors - Lou-Ella M M Alexander
  • KD Validated
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
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