验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HL-60 cells, mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51044-1-AP targets ELL in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ELL fusion protein Ag0617 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elongation factor RNA polymerase II |
| 别名 | C19orf17, ELL, ELL1, MEN |
| 计算分子量 | 500 aa, 68 kDa |
| 观测分子量 | 68-80 kDa |
| GenBank蛋白编号 | BC049195 |
| 基因名称 | ELL |
| Gene ID (NCBI) | 8178 |
| RRID | AB_2099137 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55199 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELL antibody 51044-1-AP | Download protocol |
| IHC protocol for ELL antibody 51044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Aberrant activation of non-coding RNA targets of transcriptional elongation complexes contributes to TDP-43 toxicity.
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Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Prostate ELL is an HIF-1alpha partner that regulates and responds to hypoxia response in PC3 cells.
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Mol Immunol Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival.
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