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HuC Polyclonal antibody

HuC Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 55047-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IP, ELISA and More (2)

ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, Neuro-2a cells
Positive IP detected inNeuro-2a cells
Positive IHC detected inhuman lung cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55047-1-AP targets HuC in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C)
别名 ELAVL 3, ELAVL3, ELAV-like protein 3, Hu antigen C, Hu-antigen C
计算分子量 40 kDa
观测分子量 36-38 kDa
GenBank蛋白编号NM_001420
基因名称 HuC
Gene ID (NCBI) 1995
RRIDAB_10859256
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14576
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members.

实验方案

Product Specific Protocols
WB protocol for HuC antibody 55047-1-APDownload protocol
IHC protocol for HuC antibody 55047-1-APDownload protocol
IP protocol for HuC antibody 55047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Acta Neuropathol

Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.

Authors - Sandra Diaz-Garcia
humanWB,IF,RIP

Nucleic Acids Res

HuB and HuD repress telomerase activity by dissociating HuR from TERC.

Authors - Xiaolei Cheng
zebrafishIF

Invest Ophthalmol Vis Sci

Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function

Authors - Hou-Zhi Yang
humanWB

Am J Pathol

Anaplastic lymphoma kinase (ALK) overexpression is associated with aggressive phenotypic characteristics of ovarian high-grade serous carcinoma.

Authors - Toshihide Matsumoto
humanIF

Cell Stem Cell

KCNJ2 inhibition mitigates mechanical injury in a human brain organoid model of traumatic brain injury

Authors - Jesse D Lai
humanWB

iScience

Multivalent GU-rich oligonucleotides sequester TDP-43 in the nucleus by inducing high molecular weight RNP complexes

Authors - Xi Zhang
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