验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, PC-12 cells, Neuro-2a cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67097-1-Ig targets ELAVL2 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 文献引用反应性 | human, mouse |
| 免疫原 | ELAVL2 fusion protein Ag28310 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) |
| 别名 | Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2, ELAV2 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC030692 |
| 基因名称 | ELAVL2 |
| Gene ID (NCBI) | 1993 |
| RRID | AB_2882402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q12926 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELAVL2 antibody 67097-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Sci Adv The long noncoding RNA mimi scaffolds neuronal granules to maintain nervous system maturity | ||
Mol Neurodegener Increased expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) contributes to synapse loss in Alzheimer's disease |
