验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15737-1-AP targets ELA3A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag8536 Product name: Recombinant human ELA3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-270 aa of BC008383 Sequence: SSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSGFGCNFIWKPTVFTRVSAFIDWIEETIASH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elastase 3A, pancreatic |
| 别名 | CELA3A, Chymotrypsin-like elastase family member 3A, EC:3.4.21.70, ELA3, Elastase 3A |
| 计算分子量 | 270 aa, 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC008383 |
| 基因名称 | ELA3A |
| Gene ID (NCBI) | 10136 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09093 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELA3A, also known as CELA3A (Chymotrypsin Like Elastase 3A), is synthesized in the pancreas and secreted into the intestine as an inactive precursor, or zymogen, to prevent it from digesting the pancreas itself. Structurally, the mature protein is 270 amino acids long with a molecular mass of approximately 29.5 kDa . It contains a conserved trypsin-like serine protease domain (Peptidase S1) characteristic of its function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELA3A antibody 15737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
