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验证数据展示

  • WB analysis of mouse brain using 17069-1-AP

    WB analysis of mouse brain using 17069-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17069-1-AP (EIF5A2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 17069-1-AP

    IHC staining of rat colon using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of human prostate cancer using 17069-1-AP

    IHC staining of human prostate cancer using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of human prostate cancer using 17069-1-AP

    IHC staining of human prostate cancer using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IP experiment of mouse brain using 17069-1-AP

    IP experiment of mouse brain using 17069-1-AP

    IP result of anti-EIF5A1/EIF5A2 (IP:17069-1-AP, 3ug; Detection:17069-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 17069-1-AP

    IHC staining of mouse brain using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of mouse colon using 17069-1-AP

    IHC staining of mouse colon using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of human ovary cancer using 17069-1-AP

    IHC staining of human ovary cancer using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of human placenta using 17069-1-AP

    IHC staining of human placenta using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of mouse kidney using 17069-1-AP

    IHC staining of mouse kidney using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of mouse ovary using 17069-1-AP

    IHC staining of mouse ovary using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of rat colon using 17069-1-AP

    IHC staining of rat colon using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of rat kidney using 17069-1-AP

    IHC staining of rat kidney using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of rat ovary using 17069-1-AP

    IHC staining of rat ovary using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of mouse ovary using 17069-1-AP

    IHC staining of mouse ovary using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • IHC staining of rat ovary using 17069-1-AP

    IHC staining of rat ovary using 17069-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 17069-1-AP (EIF5A1/EIF5A2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF5A1/EIF5A2 antibody included
  • Featured Product
  • KD/KO Validated

EIF5A1/EIF5A2 Polyclonal antibody

EIF5A1/EIF5A2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17069-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (2)

别名

EIF5A2, EIF 5A2, eIF-5A2, eIF-5A-2, eIF5AII

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, A2780 cells, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat colon tissue, human colon cancer tissue, human stomach cancer tissue, mouse kidney tissue, human ovary cancer tissue, human prostate cancer tissue, rat ovary tissue, mouse colon tissue, mouse ovary tissue, rat kidney tissue, human placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF5A1/EIF5A2 Ready-To-Use IHC Kit. EIF5A1/EIF5A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17069-1-AP targets EIF5A1/EIF5A2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag10895

Product name: Recombinant human EIF5A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-153 aa of BC036072

Sequence: MADEIDFTTGDAGASSTYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIKPCK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A2
别名 EIF5A2, EIF 5A2, eIF-5A2, eIF-5A-2, eIF5AII
计算分子量 153 aa, 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC036072
基因名称 EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2262009
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 5A (EIF5A) plays an essential role in the viability of eukaryotic cells. EIF5A is known to act as a translation initiation factor specific for a small number of mRNAs, a cellular target of HIV-1 REV protein, and an exportin-4-dependent nuclear export cargo. It is also involved in mRNA turnover and the establishment of actin polarity. [PMID:16157662]. EIF5A2, one isoform of EIF5A, has a key at the level of mRNA turnoverby acting downstream of decapping. It also involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity [PMID:14622290]. EIF5A2 shares 84% identity of amino acid sequence with EIF5A1 isoform, so EIF5A2 poly-antibody could recognize both EIF5A2 and EIF5A1.

实验方案

Product Specific Protocols
IHC protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
IP protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
WB protocol for EIF5A1/EIF5A2 antibody 17069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

Nat Commun

Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr

Authors - Saeede Salehi
View Article

Adv Sci (Weinh)

eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR-Mediated Metabolic Rewiring

Authors - Sonam Mittal
View Article
humanWB

Cell Mol Biol Lett

SUMOylation modulates eIF5A activities in both yeast and pancreatic ductal adenocarcinoma cells

Authors - Rocío Seoane
View Article
mouseWB,RIP

Oncogene

Identification of genes that promote PI3K pathway activation and prostate tumour formation

Authors - Jeffrey C Francis
View Article
mouseWB

PLoS Biol

Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.

Authors - Dong Ma
View Article
mouseWB

Neurobiol Dis

Progressive alterations in polysomal architecture and activation of ribosome stalling relief factors in a mouse model of Huntington's disease

Authors - Eva Martin-Solana
View Article
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