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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    WB analysis using 67214-1-Ig (same clone as 67214-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67214-1-Ig (same clone as 67214-1-PBS)

    IF Staining of HEK-293 using 67214-1-Ig (same clone as 67214-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67214-1-PBS in a different storage buffer formulation.

EIF5A Monoclonal antibody, PBS Only

EIF5A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67214-1-PBS

产品说明书

CloneNo. 2D1B6

宿主/亚型

Mouse / IgG1

种属反应性

rat, mouse, human

应用

WB, IF/ICC, Indirect ELISA

别名

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF5A Ready-To-Use IHC Kit. EIF5A primary antibody included.

产品信息

67214-1-PBS targets EIF5A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原 EIF5A fusion protein Ag28588 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A
别名 eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC001832
基因名称 EIF5A
Gene ID (NCBI) 1984
RRIDAB_2882506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP63241
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.