EIF5 Polyclonal antibody
EIF5 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11155-1-AP targets EIF5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, goat |
| Immunogen | EIF5 fusion protein Ag1629 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 5 |
| Synonyms | eIF 5, EIF 5A, EIF5 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC032866 |
| Gene Symbol | EIF5 |
| Gene ID (NCBI) | 1983 |
| RRID | AB_2877748 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55010 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic translation initiation factor 5, also named as EIF5, is a 431 amino acid protein, which contains one W2 domain and belongs to the eIF-2-beta/eIF-5 family. EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. EIF5 plays multiple roles in translation initiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF5 antibody 11155-1-AP | Download protocol |
| IHC protocol for EIF5 antibody 11155-1-AP | Download protocol |
| IF protocol for EIF5 antibody 11155-1-AP | Download protocol |
| IP protocol for EIF5 antibody 11155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats | ||
Nucleic Acids Res An interaction between eIF4A3 and eIF3g drives the internal initiation of translation | ||
Front Oncol CircRNA-100338 Is Associated With mTOR Signaling Pathway and Poor Prognosis in Hepatocellular Carcinoma. |
