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验证数据展示

  • IHC staining of human prostate cancer using 11182-1-AP

    IHC staining of human prostate cancer using 11182-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11182-1-AP (EIF4EBP2 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of mouse testis using 11182-1-AP

    WB analysis of mouse testis using 11182-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11182-1-AP (EIF4EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

EIF4EBP2 Polyclonal antibody

EIF4EBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11182-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11182-1-AP targets EIF4EBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性pig
免疫原

CatNo: Ag1670

Product name: Recombinant human EIF4EBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of BC050633

Sequence: MSSSAGSGHQPSQSRAIPTRTVAISDAAQLPHDYCTTPGGTLFSTTPGGTRIIYDRKFLLDRRNSPMAQTPPCHLPNIPGVTSPGTLIEDSKVEVNNLNNLNNHDRKHAVGDDAQFEMDI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 2
别名 Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2
计算分子量 13 kDa
观测分子量 ~20 kDa
GenBank蛋白编号BC050633
基因名称 EIF4EBP2
Gene ID (NCBI) 1979
RRIDAB_2877750
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13542
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
IHC protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Cell Mol Life Sci

Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs

Authors - Xiaolong Yuan
View Article