EIF4A2 Monoclonal antibody
EIF4A2 Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Rat, Mouse
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2D11E11
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
Positive IF detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67799-1-Ig targets EIF4A2 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Applications | IF/ICC, WB, ELISA |
Tested Reactivity | Human, Rat, Mouse |
Immunogen | EIF4A2 fusion protein Ag9085 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 4A, isoform 2 |
Synonyms | BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F |
Calculated Molecular Weight | 408 aa, 46 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC012547 |
Gene Symbol | EIF4A2 |
Gene ID (NCBI) | 1974 |
RRID | AB_2918563 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14240 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
实验方案
Product Specific Protocols | |
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WB protocol for EIF4A2 antibody 67799-1-Ig | Download protocol |
IF protocol for EIF4A2 antibody 67799-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |