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验证数据展示

  • WB analysis using 84131-5-RR (same clone as 84131-5-PBS)

    WB analysis using 84131-5-RR (same clone as 84131-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84131-5-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84131-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84131-5-RR (same clone as 84131-5-PBS)

    FC experiment of HeLa using 84131-5-RR (same clone as 84131-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-5-RR, Clone:240894A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84131-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84131-5-RR

    Affinity and Kinetic Characterization of 84131-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84131-5-RR against Human EIF3F were performed. The affinity constant is 0.474 nM.

EIF3F Recombinant monoclonal antibody, PBS Only

EIF3F Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84131-5-PBS

产品说明书

CloneNo. 240894A6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

240894A6, eIF3 p47

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84131-5-PBS targets EIF3F in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34849

Product name: Recombinant human EIF3F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 105-357 aa of BC000490

Sequence: SYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit F
别名 240894A6, eIF3 p47
计算分子量 38 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC000490
基因名称 EIF3F
Gene ID (NCBI) 8665
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO00303
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.

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