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EIF3D Monoclonal antibody

EIF3D Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C5A3

Cat No : 66024-1-Ig

Print datasheet

Synonyms

eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7

验证数据展示

  • WB analysis of HepG2 using 66024-1-Ig

    WB analysis of HepG2 using 66024-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66024-1-Ig (EIF3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66024-1-Ig

    IHC staining of human brain using 66024-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IHC staining of human brain using 66024-1-Ig

    IHC staining of human brain using 66024-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IF Staining of HeLa using 66024-1-Ig

    IF Staining of HeLa using 66024-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66024-1-Ig(EIF3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66024-1-Ig is EIF3D Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen EIF3D fusion protein Ag18092 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit D
Synonyms eIF 3 zeta, eIF3 p66, eIF3 zeta, EIF3D, EIF3S7
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
RRIDAB_11042451
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

实验方案

Product Specific Protocols
WB protocol for EIF3D antibody 66024-1-IgDownload protocol
IHC protocol for EIF3D antibody 66024-1-IgDownload protocol
IF protocol for EIF3D antibody 66024-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols