验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, K-562 cells, HeLa cell |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Planning an IHC experiment? We recommend our IHCeasy EIF3A Ready-To-Use IHC Kit. EIF3A primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67713-1-Ig targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag24762 Product name: Recombinant human EIF3A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 266-437 aa of BC114429 Sequence: PPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTIL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 3, subunit A |
| 别名 | eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32 |
| 计算分子量 | 1382 aa, 166 kDa |
| 观测分子量 | 166 kDa |
| GenBank蛋白编号 | BC114429 |
| 基因名称 | EIF3A |
| Gene ID (NCBI) | 8661 |
| RRID | AB_2882903 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14152 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| IHC protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| WB protocol for EIF3A antibody 67713-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Patient-driven discovery of CCN1 to rescue cutaneous wound healing in diabetes via the intracellular EIF3A/CCN1/ATG7 signaling by nanoparticle-enabled delivery | ||
Cell Prolif G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration | ||
Cell Death Differ Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression | ||
ACS Nano Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels |
