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验证数据展示

  • WB analysis of BxPC-3 using 27665-1-AP

    WB analysis of BxPC-3 using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27665-1-AP

    IF Staining of HepG2 using 27665-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27665-1-AP (EIF3A antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 27665-1-AP

    WB analysis using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27665-1-AP

    IHC staining of human lung cancer using 27665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3A antibody included

EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27665-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, SKOV-3 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3A Ready-To-Use IHC Kit. EIF3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27665-1-AP targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag24752

Product name: Recombinant human EIF3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 266-437 aa of BC114429

Sequence: PPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit A
别名 eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32
计算分子量 1382 aa, 166 kDa
观测分子量 166 kDa
GenBank蛋白编号BC114429
基因名称 EIF3A
Gene ID (NCBI) 8661
RRIDAB_2880942
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for EIF3A antibody 27665-1-APDownload protocol
IHC protocol for EIF3A antibody 27665-1-APDownload protocol
WB protocol for EIF3A antibody 27665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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