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EIF2C3-Specific Polyclonal antibody

EIF2C3-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19692-1-AP

Print datasheet

Synonyms

AGO3, Argonaute 3, Argonaute3, eIF 2C 3, eIF2C 3, EIF2C3, EIF2C3-Specific, hAgo3, Protein argonaute 3

验证数据展示

  • IHC staining of human stomach cancer using 19692-1-AP

    IHC staining of human stomach cancer using 19692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2C3 antibody included
  • IHC staining of human stomach cancer using 19692-1-AP

    IHC staining of human stomach cancer using 19692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19692-1-AP (EIF2C3-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2C3 antibody included
  • WB analysis of SKOV-3 using 19692-1-AP

    WB analysis of SKOV-3 using 19692-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19692-1-AP (EIF2C3-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSKOV-3 cells, HepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF2C3 Ready-To-Use IHC Kit. EIF2C3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19692-1-AP targets EIF2C3-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2C, 3
Synonyms AGO3, Argonaute 3, Argonaute3, eIF 2C 3, eIF2C 3, EIF2C3, EIF2C3-Specific, hAgo3, Protein argonaute 3
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberNM_024852
Gene Symbol EIF2C3
Gene ID (NCBI) 192669
RRIDAB_2878597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9G7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2C3, also named as AGO3, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi). EIF2C3 binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. EIF2C3 lacks endonuclease activity and does not appear to cleave target mRNAs. This antibody is specific to EIF2C3.

实验方案

Product Specific Protocols
WB protocol for EIF2C3-Specific antibody 19692-1-APDownload protocol
IHC protocol for EIF2C3-Specific antibody 19692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

A novel class of microRNA-recognition elements that function only within open reading frames.

Authors - Kai Zhang
View Article