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AGO2 Polyclonal antibody

AGO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 10686-1-AP

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Synonyms

AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10



经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy AGO2/EIF2C2 Ready-To-Use IHC Kit. AGO2/EIF2C2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10686-1-AP targets AGO2 in WB, IP, RIP, CLIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IP, RIP, CLIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, fish
Immunogen AGO2 fusion protein Ag1032 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2C, 2
Synonyms AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10
Calculated Molecular Weight 97 kDa
Observed Molecular Weight94-97 kDa
GenBank Accession NumberBC007633
Gene Symbol EIF2C2
Gene ID (NCBI) 27161
RRIDAB_2096299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions. This antibody is specific to EIF2C2. EIF2C2 exists two isoforms and molecular weight of isoforms are 97 kDa and 94 kDa.

实验方案

Product Specific Protocols
WB protocol for AGO2 antibody 10686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Med

Cancer cells exploit an orphan RNA to drive metastatic progression.

Authors - Lisa Fish
humanIP

Mol Cancer

PMID: 32727463

Authors - Cefan Zhou
humanWB

Nat Commun

Liver governs adipose remodelling via extracellular vesicles in response to lipid overload.

Authors - Yue Zhao
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma
humanRIP

J Hematol Oncol

LINC01123, a c-Myc-activated long non-coding RNA, promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis.

Authors - Qian Hua
RIP

Clin Transl Med

LncRNA nuclear-enriched abundant transcript 1 shuttled by prostate cancer cells-secreted exosomes initiates osteoblastic phenotypes in the bone metastatic microenvironment via miR-205-5p/runt-related transcription factor 2/splicing factor proline- and glutamine-rich/polypyrimidine tract-binding protein 2 axis.

Authors - Chengqiang Mo