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验证数据展示

  • WB analysis using 29935-1-AP

    WB analysis using 29935-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29935-1-AP (EIF2B5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 29935-1-AP

    IF Staining of MCF-7 using 29935-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 29935-1-AP

    IF Staining of MCF-7 using 29935-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 29935-1-AP

    IHC staining of human colon cancer using 29935-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2B5 antibody included
  • IHC staining of human colon cancer using 29935-1-AP

    IHC staining of human colon cancer using 29935-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF2B5 antibody included

EIF2B5 Polyclonal antibody

EIF2B5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29935-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CACH, CLE, EIF 2B, EIF2B5, EIF2BE, EIF2Bepsilon, LVWM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, HT-29 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF2B5 Ready-To-Use IHC Kit. EIF2B5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29935-1-AP targets EIF2B5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag32175

Product name: Recombinant human EIF2B5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 455-711 aa of NM_003907.2

Sequence: DAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
别名 CACH, CLE, EIF 2B, EIF2B5, EIF2BE, EIF2Bepsilon, LVWM
计算分子量80 kDa
观测分子量65-80 kDa
GenBank蛋白编号NM_003907.2
基因名称 EIF2B5
Gene ID (NCBI) 8893
RRIDAB_2923623
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).

实验方案

Product Specific Protocols
WB protocol for EIF2B5 antibody 29935-1-APDownload protocol
IHC protocol for EIF2B5 antibody 29935-1-APDownload protocol
IF protocol for EIF2B5 antibody 29935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols