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验证数据展示

  • WB analysis using 86578-1-RR (same clone as 86578-1-PBS)

    WB analysis using 86578-1-RR (same clone as 86578-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86578-1-RR (EIF2B2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 86578-1-RR (same clone as 86578-1-PBS)

    IP experiment of A431 using 86578-1-RR (same clone as 86578-1-PBS)

    IP result of anti-EIF2B2 (IP:86578-1-RR, 4ug; Detection:86578-1-RR 1:3000) with A431 cells lysate 1000 ug. This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 86578-1-RR (same clone as 86578-1-PBS)

    IHC staining of rat brain using 86578-1-RR (same clone as 86578-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86578-1-RR (EIF2B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86578-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86578-1-RR

    Affinity and Kinetic Characterization of 86578-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86578-1-RR against Human EIF2B2 were performed. The affinity constant is 18.7 nM.

EIF2B2 Recombinant monoclonal antibody, PBS Only

EIF2B2 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 86578-1-PBS

产品说明书

CloneNo. 251505G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

EIF 2Bbeta, EIF2B, EIF2B 2, eIF2B GDP-GTP exchange factor subunit beta, EIF2BB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86578-1-PBS targets EIF2B2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1502

Product name: Recombinant human EIF2B2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC011750

Sequence: MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
别名 EIF 2Bbeta, EIF2B, EIF2B 2, eIF2B GDP-GTP exchange factor subunit beta, EIF2BB
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC011750
基因名称 EIF2B2
Gene ID (NCBI) 8892
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49770
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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