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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

    WB analysis using 66646-1-Ig (same clone as 66646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK2 antibody included
  • IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    IHC staining of human testis using 66646-1-Ig (same clone as 66646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66646-1-Ig (EIF2AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66646-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK2 antibody included

EIF2AK2, PKR Monoclonal antibody, PBS Only

EIF2AK2, PKR Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66646-1-PBS

产品说明书

CloneNo. 2A8A4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EIF2AK2, PKR, 2A8A4, EC:2.7.10.2, EC:2.7.11.1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF2AK2 Ready-To-Use IHC Kit. EIF2AK2 primary antibody included.

产品信息

66646-1-PBS targets EIF2AK2, PKR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13010

Product name: Recombinant human EIF2AK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-373 aa of BC093676

Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 2
别名 EIF2AK2, PKR, 2A8A4, EC:2.7.10.2, EC:2.7.11.1
计算分子量 551 aa, 62 kDa
观测分子量 65-68 kDa
GenBank蛋白编号BC093676
基因名称 EIF2AK2
Gene ID (NCBI) 5610
RRIDAB_2882004
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP19525
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2, also known as PKR or PRKR) is an important component of the host innate immuneantiviral response.The product of the HSAEIF2AK2 gene is a 68 kDa protein that is ubiquitously expressed at low levels. The N-terminal part of this protein contains two dsRNA binding motifs (dsRBM) .The C-terminal EIF2S1 kinase domain includes 12 sub-domains that are conserved in several Species(PMID:16954046 ).This antibody is specific to EIF2AK2.