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验证数据展示

  • WB analysis of HEK-293 using 20499-1-AP (same clone as 20499-1-PBS)

    WB analysis of HEK-293 using 20499-1-AP (same clone as 20499-1-PBS)

    WB result of EIF2AK1 antibody (20499-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

  • WB analysis using 20499-1-AP (same clone as 20499-1-PBS)

    WB analysis using 20499-1-AP (same clone as 20499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20499-1-AP (EIF2AK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

    IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK1 antibody included
  • IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

    IHC staining of human breast cancer using 20499-1-AP (same clone as 20499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20499-1-AP (EIF2AK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF2AK1 antibody included
  • IF Staining of HeLa using 20499-1-AP (same clone as 20499-1-PBS)

    IF Staining of HeLa using 20499-1-AP (same clone as 20499-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2AK1 antibody (20499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20499-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EIF2AK1 Polyclonal antibody, PBS Only

EIF2AK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20499-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF2AK1 Ready-To-Use IHC Kit. EIF2AK1 primary antibody included.

产品信息

20499-1-PBS targets EIF2AK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14236

Product name: Recombinant human EIF2AK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-630 aa of BC006524

Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 1
别名 EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor, Heme regulated inhibitor
计算分子量 630 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC006524
基因名称 EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_10697665
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQI3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.

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