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EIF1AX Polyclonal antibody

EIF1AX Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14654-1-AP

Print datasheet

Synonyms

eIF 1A, eIF 1A X isoform, eIF 4C, EIF1A, EIF1AP1, EIF1AX, EIF4C

验证数据展示

  • WB analysis using 14654-1-AP

    WB analysis using 14654-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis of HepG2 using 14654-1-AP

    WB analysis of HepG2 using 14654-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14654-1-AP (EIF1AX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14654-1-AP

    IF Staining of HepG2 using 14654-1-AP

    Immunofluorescent analysis of HepG2 cells using 14654-1-AP (EIF1AX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A375 cells, HeLa cells, PC-3 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14654-1-AP targets EIF1AX in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen EIF1AX fusion protein Ag6253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 1A, X-linked
Synonyms eIF 1A, eIF 1A X isoform, eIF 4C, EIF1A, EIF1AP1, EIF1AX, EIF4C
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16-23 kDa
GenBank Accession NumberBC067851
Gene Symbol EIF1AX
Gene ID (NCBI) 1964
RRIDAB_2878072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 1A, X-chromosome(EIF1AX), designated as to distinguish from the Y-linked homolog, has an unblocked N-terminal proline, which was consistent with the general pattern of eukaryotic protein. It's may be required for maximal rate of protein biosynthesis. Like EIF4C, it enhances ribosome dissociation into subunits and stabilizeds the binding of the initiator Met-tRNA(l) to 40S ribosomal subunits.

实验方案

Product Specific Protocols
WB protocol for EIF1AX antibody 14654-1-APDownload protocol
IF protocol for EIF1AX antibody 14654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Authors - Hao-Yu Li
View Article