验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, A549 cells, HEK-293 cells, HL-60 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20528-1-AP targets EIF1AD in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14390 Product name: Recombinant human EIF1AD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC005131 Sequence: MSQATKRKHVVKEVLGEHIVPSNQQQIVRVLRTPGNNLHEVETAQGQRFLVSMPSKYRKNIWIKRGDFLIVDPIEEGEKVKAEISFVLCKDHVRSLQKEGFWPEAFSEVAEKHNNRNRQTQPELPAEPQLSGEESSSEDDSDLFVNTNRRQYHESEEESEEEEAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 1A domain containing |
| 别名 | EIF1AD, Haponin |
| 计算分子量 | 165 aa, 19 kDa |
| 观测分子量 | 27 kDa |
| GenBank蛋白编号 | BC005131 |
| 基因名称 | EIF1AD |
| Gene ID (NCBI) | 84285 |
| RRID | AB_10693533 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N9N8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF1AD antibody 20528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Structural basis for the final steps of human 40S ribosome maturation.
| ||
Elife The final step of 40S ribosomal subunit maturation is controlled by a dual key lock. | ||
Elife Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36. | ||
Life Sci Alliance CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau |
