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EI24 Polyclonal antibody

EI24 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20456-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

EI24, etoposide induced 2.4 mRNA, p53 induced gene 8 protein, p53-induced factor Ei24, PIG8, TP53I8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inK-562 cells, HT-1080 cells, HeLa cells, L02 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20456-1-AP targets EI24 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原

CatNo: Ag14169

Product name: Recombinant human EI24 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 267-340 aa of BC002390

Sequence: SILFPLFIISANEAKTPGKAYLFQLRLFSLVVFLSNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 etoposide induced 2.4 mRNA
别名 EI24, etoposide induced 2.4 mRNA, p53 induced gene 8 protein, p53-induced factor Ei24, PIG8, TP53I8
计算分子量 340 aa, 39 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC002390
基因名称 EI24
Gene ID (NCBI) 9538
RRIDAB_10858326
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14681
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ei24 is an ER-localized protein originally identified as a p53-induced proapoptotic gene in etoposide-treated NIH3T3 cells. It has been shown to be able to bind to the antiapoptotic protein Bcl-2 (PMID: 15781622), play a role in autophagy (PMID: 23074225), and induce proliferate arrest/apoptosis (PMID: 10594026). Ei24 can bind specifically to IMPβ1 and IMPα2 to impede their normal role in nuclear import (PMID: 24821838).

实验方案

Product Specific Protocols
WB protocol for EI24 antibody 20456-1-APDownload protocol
IHC protocol for EI24 antibody 20456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Int J Biochem Cell Biol

Etoposide-induced protein 2.4 ameliorates high glucose-induced epithelial-mesenchymal transition by activating adenosine monophosphate-activated protein kinase pathway in renal tubular cells

Authors - Fan Li
  • KD Validated
humanWB

BMC Cancer

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Authors - Yue Shi
  • KD Validated
humanWB,IHC

Expert Rev Mol Diagn

Prognostic role and immune infiltration characteristics of EI24 in multiple cancer types

Authors - Wanli Yang
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