验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29303-1-AP targets EHMT2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag29160 Product name: Recombinant human EHMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-265 aa of BC018718 Sequence: MSDDVHSLGKVTSDLAKRRKLNSGGGLSEELGSARRSGEVTLTKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQLSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | euchromatic histone-lysine N-methyltransferase 2 |
| 别名 | BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a |
| 计算分子量 | 1210 aa, 132 kDa |
| 观测分子量 | 160-180 kDa |
| GenBank蛋白编号 | BC018718 |
| 基因名称 | EHMT2 |
| Gene ID (NCBI) | 10919 |
| RRID | AB_2918275 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96KQ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EHMT2 antibody 29303-1-AP | Download protocol |
| IHC protocol for EHMT2 antibody 29303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication | ||
Oncogenesis Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.
| ||
Mol Biotechnol Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development
| ||
Mol Carcinog A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer |
