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验证数据展示

  • IP experiment of K-562 using 55436-1-AP (same clone as 55436-1-PBS)

    IP experiment of K-562 using 55436-1-AP (same clone as 55436-1-PBS)

    IP result of anti-EHMT1 (IP:55436-1-AP, 4ug; Detection:55436-1-AP 1:500) with K-562 cells lysate 1360 ug. This data was developed using the same antibody clone with 55436-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 55436-1-AP (same clone as 55436-1-PBS)

    IF Staining of HeLa using 55436-1-AP (same clone as 55436-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EHMT1 antibody (55436-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55436-1-PBS in a different storage buffer formulation.

EHMT1 Polyclonal antibody, PBS Only

EHMT1 Polyclonal Antibody for IF/ICC, IP, Indirect ELISA
Cat No. 55436-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55436-1-PBS targets EHMT1 in IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 euchromatic histone-lysine N-methyltransferase 1
别名
计算分子量 141 kDa
观测分子量150-170 kDa
GenBank蛋白编号NM_024757
基因名称 EHMT1
Gene ID (NCBI) 79813
RRIDAB_3670252
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9B1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EHMT1, also known as GLP (G9a-like protein), encodes a histone lysine methyltransferase that controls brown adipose cell fate, adaptive thermogenesis, and glucose homeostasis in vivo (PMID: 24196706). EHMTs, along with LSD1 (an H3K4 demethylase), are components of the REST complex, which silences neuronal pathway genes in non-neuronal cells. EHMT1 is mainly responsible for the monomethylation and dimethylation of histone H3 lysine 9 (H3K9), forming a heteromeric complex with EHMT2 (G9a) in euchromatin (PMID: 34214254, 37663929).

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