验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue |
| Positive IHC detected in | mouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25320-1-AP targets EHD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
CatNo: Ag18216 Product name: Recombinant human EHD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 353-422 aa of BC156996 Sequence: FPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EH-domain containing 3 |
| 别名 | EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3 |
| 计算分子量 | 535 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC156996 |
| 基因名称 | EHD3 |
| Gene ID (NCBI) | 30845 |
| RRID | AB_2880029 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZN3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for EHD3 antibody 25320-1-AP | Download protocol |
| WB protocol for EHD3 antibody 25320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
| ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.
| ||
EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. | ||
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Int J Mol Sci Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases | ||
Am J Physiol Renal Physiol Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney |
