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验证数据展示

  • WB analysis using 31098-1-AP

    WB analysis using 31098-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31098-1-AP (EGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EGR1 Polyclonal antibody

EGR1 Polyclonal Antibody for WB, ELISA
Cat No. 31098-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

AT225, early growth response 1, EGR 1, EGR1, G0S30, KROX 24, KROX24, NGFI A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, Zinc finger protein 225, Zinc finger protein Krox 24, ZNF225

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31098-1-AP targets EGR1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 EGR1 fusion protein Ag33440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 early growth response 1
别名 AT225, early growth response 1, EGR 1, EGR1, G0S30, KROX 24, KROX24, NGFI A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, Zinc finger protein 225, Zinc finger protein Krox 24, ZNF225
计算分子量 543 aa, 58 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC073983
基因名称 EGR1
Gene ID (NCBI) 1958
RRIDAB_3669855
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP18146
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGR1, also named as Early growth response protein 1, is a 543 amino acid protein, which belongs to the EGR C2H2-type zinc-finger protein family. EGR1 as a transcriptional regulator recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes and binds double-stranded target DNA, irrespective of the cytosine methylation status. EGR1 regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. EGR1 activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation and plays a role in the regulation of cell survival, proliferation and cell death. Egr1 is suggested to be a 55-kDa protein according to the translation of its coding sequence. Analysis of cytoplasmic and nuclear fractions of normal and irradiated native CNS tissue by Western blotting revealed a 110-kDa band for Egr1 localized in the cytoplasm and a 75-kDa version for the nuclear Egr1(PMID: 17497096)

实验方案

Product Specific Protocols
WB protocol for EGR1 antibody 31098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols