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验证数据展示

  • WB analysis using 27875-1-AP

    WB analysis using 27875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27875-1-AP

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EGLN3 antibody included
  • IHC staining of mouse colon using 27875-1-AP

    IHC staining of mouse colon using 27875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27875-1-AP (EGLN3/PHD3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EGLN3 antibody included

EGLN3 Polyclonal antibody

EGLN3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27875-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EGLN3 Ready-To-Use IHC Kit. EGLN3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27875-1-AP targets EGLN3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 EGLN3 fusion protein Ag27464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 egl nine homolog 3 (C. elegans)
别名 EGLN3/PHD3, PHD3, EC:1.14.11.-, EC:1.14.11.29, Egl nine homolog 3
计算分子量 27 kDa
观测分子量27 kDa
GenBank蛋白编号BC010992
基因名称 EGLN3
Gene ID (NCBI) 112399
RRIDAB_3086001
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Z9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

实验方案

Product Specific Protocols
WB protocol for EGLN3 antibody 27875-1-APDownload protocol
IHC protocol for EGLN3 antibody 27875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Sci Rep

Mechanism of action for Troxerutin targeting the sialylation-related gene EGLN3 for the treatment of LUAD

Authors - Yanan Wu
  • KD Validated
View Article