验证数据展示
经过测试的应用
| Positive WB detected in | Calu-1 cells, A431 cells, A549 cells, HeLa cells, HeLa/RPE1 cells, HepG2 cells, MCF-7 cells, NCI-H1299 cells, L02 cells, NCL-H1299 cells |
| Positive IP detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18986-1-AP targets EGFR-Specific in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, bovine, goat, sheep, sus scrofa (pig) |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| 别名 | EGFR, EGF R, EGF Receptor , Epidermal growth factor receptor, Proto-oncogene c-ErbB-1 |
| 计算分子量 | 134 kDa |
| 观测分子量 | 145-165 kDa |
| GenBank蛋白编号 | NM_005228 |
| 基因名称 | EGFR |
| Gene ID (NCBI) | 1956 |
| RRID | AB_10596476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00533 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ERBB1, also named as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for EGFR-Specific antibody 18986-1-AP | Download protocol |
| WB protocol for EGFR-Specific antibody 18986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Micropeptide MIAC inhibits the tumor progression by interacting with AQP2 and inhibiting EREG/EGFR signaling in renal cell carcinoma | ||
Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.
| ||
Immunity The cGAS-STING pathway activates transcription factor TFEB to stimulate lysosome biogenesis and pathogen clearance | ||
Exploration (Beijing) Targeting MGAT4A-Mediated N-Glycosylation as a Therapeutic Strategy to Inhibit Glioblastoma Stem Cell Invasion. | ||
Adv Mater Dual Single-Atom Engineered NADH Oxidase-Mimic Boosts In Situ NAD(+) Biotransformation for Targeted Myocardial Ischemia/Reperfusion Protection. | ||
Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma |
