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Pikachurin Polyclonal antibody

Pikachurin Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14578-1-AP

Print datasheet

Synonyms

Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin

验证数据展示

  • WB analysis of human placenta using 14578-1-AP

    WB analysis of human placenta using 14578-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14578-1-AP (Pikachurin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 14578-1-AP

    IF Staining of SH-SY5Y using 14578-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 14578-1-AP (Pikachurin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, PC-3 cells, SH-SY5Y cells
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14578-1-AP targets Pikachurin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Pikachurin fusion protein Ag5986 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGF-like, fibronectin type III and laminin G domains
Synonyms Agrin like protein, AGRINL, AGRNL, EGFLAM, Pikachurin
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC033177
Gene Symbol EGFLAM
Gene ID (NCBI) 133584
RRIDAB_2277653
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ63HQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This antibody detects a 60-kDa band, which has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).

实验方案

Product Specific Protocols
WB protocol for Pikachurin antibody 14578-1-APDownload protocol
IF protocol for Pikachurin antibody 14578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Stem Cells Dev

Pikachurin Is Partially Involved in the Synaptic Connection Between Donor and Host Cells in Late-Stage rd1 Mice Following Conspecific Photoreceptor Transplantation.

Authors - Chen Liang
View Article