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验证数据展示

  • IHC staining of human kidney using 24729-1-AP

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 24729-1-AP

    IHC staining of human kidney using 24729-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24729-1-AP (EFTUD1 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis using 24729-1-AP

    WB analysis using 24729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24729-1-AP (EFTUD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

EFTUD1 Polyclonal antibody

EFTUD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24729-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EFL1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Elongation factor-like GTPase 1, FAM42A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24729-1-AP targets EFTUD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20371

Product name: Recombinant human EFTUD1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 772-1120 aa of BC156743

Sequence: LIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elongation factor Tu GTP binding domain containing 1
别名 EFL1, Elongation factor Tu GTP-binding domain-containing protein 1, Elongation factor-like 1, Elongation factor-like GTPase 1, FAM42A
计算分子量 1120 aa, 125 kDa
观测分子量 125-135 kDa
GenBank蛋白编号BC156743
基因名称 EFTUD1
Gene ID (NCBI) 79631
RRIDAB_2879693
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z2Z2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EFTUD1 (Elongation Factor Tu GTP Binding Domain Containing Protein 1) is a protein involved in ribosome biogenesis and translation activation. It is a member of the GTP-binding elongation factor family. EFTUD1 plays a crucial role in the release of eukaryotic translation initiation factor 6 (eIF6) from 60S ribosome subunits, allowing these subunits to join with 40S subunits to form actively translating 80S complexes. (PMID: 25015090)

实验方案

Product Specific Protocols
IHC protocol for EFTUD1 antibody 24729-1-APDownload protocol
WB protocol for EFTUD1 antibody 24729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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