验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, human placenta tissue |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
20538-1-AP targets EFS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14565 Product name: Recombinant human EFS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 100-400 aa of BC034246 Sequence: TPKPPAELEPDLEWEGGREPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTLAQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGPKVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEALSPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDTLGR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | embryonal Fyn-associated substrate |
| 别名 | CAS3, CASS3, EFS, EFS1, EFS2, HEFS, SIN |
| 计算分子量 | 561 aa, 59 kDa |
| 观测分子量 | 80-90 kDa, 67-70 kDa |
| GenBank蛋白编号 | BC034246 |
| 基因名称 | EFS |
| Gene ID (NCBI) | 10278 |
| RRID | AB_10695758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43281 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for EFS antibody 20538-1-AP | Download protocol |
| IHC protocol for EFS antibody 20538-1-AP | Download protocol |
| IP protocol for EFS antibody 20538-1-AP | Download protocol |
| WB protocol for EFS antibody 20538-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Genet LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia. | ||
Ecotoxicol Environ Saf Potential involvement of connective tissue growth factor in chondrocytes apoptosis of Kashin-Beck disease |
