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验证数据展示

  • WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

    WB analysis using 83264-5-RR (same clone as 83264-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83264-5-RR (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    IF Staining of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using EFHD2 antibody (83264-5-RR, Clone: 240185H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    FC experiment of A431 using 83264-5-RR (same clone as 83264-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EFHD2 Recombinant antibody (83264-5-RR, Clone:240185H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83264-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83264-5-RR

    Affinity and Kinetic Characterization of 83264-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83264-5-RR against Human EFHD2 were performed. The affinity constant is 1.42 nM.

EFHD2 Recombinant monoclonal antibody, PBS Only

EFHD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83264-5-PBS

产品说明书

CloneNo. 240185H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

240185H5, EF-hand domain-containing protein D2, Swiprosin 1, Swiprosin-1, SWS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

产品信息

83264-5-PBS targets EFHD2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35084

Product name: Recombinant human EFHD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-80 aa of BC023611

Sequence: ATDELATKLSRRLQMEGEGGGETPEQPGLNGAAAAAAGAPDEAAEALGSADCELSAKLLRRADLNQGIGEPQSPSRRVF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 EF-hand domain family, member D2
别名 240185H5, EF-hand domain-containing protein D2, Swiprosin 1, Swiprosin-1, SWS1
计算分子量27 kDa
GenBank蛋白编号BC023611
基因名称 EFHD2
Gene ID (NCBI) 79180
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96C19
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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