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验证数据展示

  • WB analysis using 20859-1-AP

    WB analysis using 20859-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20859-1-AP (EFHA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20859-1-AP

    IHC staining of human stomach cancer using 20859-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20859-1-AP (MICU2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MICU2 Polyclonal antibody

MICU2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20859-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EFHA1, EF-hand domain-containing family member A1, Calcium uptake protein 2, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20859-1-AP targets MICU2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag14830

Product name: Recombinant human EFHA1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 140-434 aa of BC034965

Sequence: QTAGCGSTFFRDLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFKLQKIINKQDDLMTVKTNETGYQEAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRFMENLLTEIQEMEFLQFSKGLSFMRKEDFAEWLLFFTNTENKDIYWKNVREKLSAGESISLDEFKSFCHFTTHLEDFAIAMQMFSLAHRPVRLAEFKRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRMHRGLWVPQHQSIQEYWKCVKKESIKGVKEVWKQAGKGLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand domain family, member A1
别名 EFHA1, EF-hand domain-containing family member A1, Calcium uptake protein 2, mitochondrial
计算分子量 434 aa, 50 kDa
观测分子量~40 kDa
GenBank蛋白编号BC034965
基因名称 MICU2
Gene ID (NCBI) 221154
RRIDAB_10695500
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYU8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MICU2 antibody 20859-1-APDownload protocol
IHC protocol for MICU2 antibody 20859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Signal

MICU1 alleviates hypobaric hypoxia-induced myocardial injury through regulating Ca2+ uptake to inhibit mitochondria-dependent apoptosis

Authors - Yao Li
View Article