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验证数据展示

  • WB analysis using 32115-1-AP

    WB analysis using 32115-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32115-1-AP (EFCAB4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 32115-1-AP

    WB analysis of LNCaP using 32115-1-AP

    WB result of EFCAB4A antibody (32115-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EFCAB4A transfected LNCaP cells.

  • Featured Product
  • KD/KO Validated

EFCAB4A Polyclonal antibody

EFCAB4A Polyclonal Antibody for WB, ELISA
Cat No. 32115-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Ca2+ Release-Activated Ca2+ (CRAC) Channel Regulator 2B, Calcium Release Activated Channel Regulator 2B, Calcium release-activated calcium channel regulator 2B, Calcium release-activated channel regulator 2B, CRAC channel regulator 2B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32115-1-AP targets EFCAB4A in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37031

Product name: Recombinant human EFCAB4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-255 aa of BC033196

Sequence: SAQGANPCRTPEETFESGGLDVQGTAGSLDEEEEEEERFHTVLEQLGVAPVLGEQRAVRTLWARLQRERPELLGSFEDVLIRASACLEEAARERDGLEQALRRRESEHEREVRALYEETEQLREQSRRPPSQNFARGERRSRLELELQIREQDLER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand calcium binding domain 4A
别名 Ca2+ Release-Activated Ca2+ (CRAC) Channel Regulator 2B, Calcium Release Activated Channel Regulator 2B, Calcium release-activated calcium channel regulator 2B, Calcium release-activated channel regulator 2B, CRAC channel regulator 2B
观测分子量50 kDa
GenBank蛋白编号BC033196
基因名称 EFCAB4A
Gene ID (NCBI) 283229
RRIDAB_3742528
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8N4Y2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EFCAB4A (EF-hand calcium-binding domain-containing protein 4A), also known as CRACR2B (calcium release-activated calcium channel regulator 2B), is a calcium-binding protein containing EF-hand structural domain. EFCAB4A is thought to play a key role in calcium bank-manipulated calcium endocytosis (SOCE) in T cells by regulating activation of CRAC (calcium release-activated calcium channel) channels. This protein may perform a similar function to EFCAB4B, which acts as a cytoplasmic calcium sensor that forms a complex with ORAI1 and STIM1 in the region of the junction between the endoplasmic reticulum and the plasma membrane. The EFCAB4A protein contains two EF-hand structural domains, which may be involved in calcium bank-manipulated calcium endocytosis.

实验方案

Product Specific Protocols
WB protocol for EFCAB4A antibody 32115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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