验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, PC-3 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24310-1-AP targets EEPD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19423 Product name: Recombinant human EEPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 223-569 aa of BC065518 Sequence: SLSLQSEDLDLPPGGPTQIISTRPSVEAFGGTRDGRPVLRLATWNLQGCSVEKANNPGVREVVCMTLLENSIKLLAVQELLDREALEKFCTELNQPTLPNIRKWKGPRGCWKAVVAEKPSNQLQKGAGYAGFLWDAAAGMELRDAGSQESSPSNGHGKLAGPSPYLGRFKVGSHDLTLVNLHLAALTLLGSENPSKNHSDGHRLASFAQTLQETLKGEKDVIILGDFGQGPDSNDYDILRKEKFHHLIPAHTFTNISTKNPQGSKSLDNIWISKSLKKVFTGHWAVVREGLTNPWIPDNWSWGGVASEHCPVLAEFYTEKDWSKKDAPRNGSGVALERSEANIKHER 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | endonuclease/exonuclease/phosphatase family domain containing 1 |
| 别名 | Endonuclease/exonuclease/phosphatase family domain-containing protein 1, HSPC107, KIAA1706 |
| 计算分子量 | 569 aa, 62 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC065518 |
| 基因名称 | EEPD1 |
| Gene ID (NCBI) | 80820 |
| RRID | AB_2879487 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L9B9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEPD1, also known as KIAA1706, is a 198 amino-acid protein belonging to the endonuclease/exonuclease/phosphatase family domain containing 11. EEPD1 is localized in the cell nucleus and may be involved in the splicing of DNA. The function of EEPD1 is still unknown and needs to be further studied.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for EEPD1 antibody 24310-1-AP | Download protocol |
| WB protocol for EEPD1 antibody 24310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Biochim Biophys Sin (Shanghai) EEPD1 attenuates radiation-induced cardiac hypertrophy and apoptosis by degrading FOXO3A in cardiomyocytes | ||
Nat Commun Myristoylated Eepd1 Enhances Lipolysis and Thermogenesis through PKA Activation to Combat Obesity | ||
Adv Sci (Weinh) EEPD1 Inhibition Unleashes Antitumor Immunity in Colorectal Cancer by Activating the cGAS-STING Pathway.
| ||
Biomaterials Sequential-targeting nanomedicine protects against acute kidney injury by modulating calcium influx and scavenging reactive oxygen species. |
