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验证数据展示

  • WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

    WB analysis using 10628-1-AP (same clone as 10628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10628-1-AP (EEFSEC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

    IHC staining of human kidney using 10628-1-AP (same clone as 10628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10628-1-AP (EEFSEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10628-1-PBS in a different storage buffer formulation.

EEFSEC Polyclonal antibody, PBS Only

EEFSEC Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10628-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10628-1-PBS targets EEFSEC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0980

Product name: Recombinant human EEFSEC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 269-569 aa of BC007933

Sequence: KLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic elongation factor, selenocysteine-tRNA-specific
别名
计算分子量 63 kDa
观测分子量65 kDa
GenBank蛋白编号BC007933
基因名称 EEFSEC
Gene ID (NCBI) 60678
RRIDAB_2293378
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57772
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon.

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