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验证数据展示

  • IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1D antibody included
  • IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    IHC staining of human pancreas cancer using 60085-2-Ig (same clone as 60085-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 60085-2-Ig (EEF1D Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1D antibody included
  • IP experiment of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    IP experiment of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    IP result of anti-EEF1D (IP:60085-2-Ig, 5ug; Detection:60085-2-Ig 1:1000) with MCF-7 cells lysate 3200ug. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 60085-2-Ig

    Affinity and Kinetic Characterization of 60085-2-Ig

    Biolayer interferometry (BLl) kinetic assays of 60085-2-Ig against Human EEF1D were performed. The Affinity Constant is 0.76 nM.

  • WB analysis of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    WB analysis of MCF-7 using 60085-2-Ig (same clone as 60085-2-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60085-2-Ig (EEF1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60085-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EEF1D Monoclonal antibody, PBS Only

EEF1D Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 60085-2-PBS

产品说明书

CloneNo. 3G4C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EEF1D Ready-To-Use IHC Kit. EEF1D primary antibody included.

产品信息

60085-2-PBS targets EEF1D in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0983

Product name: Recombinant human EEF1D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-647 aa of BC007847

Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
别名 3G4C4, Antigen NY-CO-4, EF 1 delta, EF 1D, EF1D
计算分子量 31 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC007847
基因名称 EEF1D
Gene ID (NCBI) 1936
RRIDAB_2096994
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29692
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 )

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