验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16091-1-AP targets EEF1A1/EEF1A2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish, oyster |
| 免疫原 |
CatNo: Ag6875 Product name: Recombinant human EEF1A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 109-463 aa of BC000432 Sequence: ADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation elongation factor 1 alpha 2 |
| 别名 | EEF1A2, eEF1A-2, EEF1AL, EF-1-alpha-2, Elongation factor 1-alpha 2 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 50-53 kDa |
| GenBank蛋白编号 | BC000432 |
| 基因名称 | EEF1A2 |
| Gene ID (NCBI) | 1917 |
| RRID | AB_2096979 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q05639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF1A2, also named as EEF1AL, STN and Statin-S1, belongs to the GTP-binding elongation factor family and EF-Tu/EF-1A subfamily. It promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 may be critical in the development of ovarian cancer. The MW of EEF1A2 is 50-53kd. This antibody can recognize both EEF1A1 and EEF1A2.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for EEF1A1/EEF1A2 antibody 16091-1-AP | Download protocol |
| IP protocol for EEF1A1/EEF1A2 antibody 16091-1-AP | Download protocol |
| WB protocol for EEF1A1/EEF1A2 antibody 16091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Oncol SNX16 activates c-Myc signaling by inhibiting ubiquitin-mediated proteasomal degradation of eEF1A2 in colorectal cancer development. | ||
Pharmacol Res Nannocystin ax, an eEF1A inhibitor, induces G1 cell cycle arrest and caspase-independent apoptosis through cyclin D1 downregulation in colon cancer in vivo. | ||
Sci Rep Biallelic mutations in the gene encoding eEF1A2 cause seizures and sudden death in F0 mice.
| ||
Br J Cancer Dissecting the expression of EEF1A1/2 genes in human prostate cancer cells: the potential of EEF1A2 as a hallmark for prostate transformation and progression. | ||
Hum Mol Genet Recapitulation of the EEF1A2 D252H neurodevelopmental disorder-causing missense mutation in mice reveals a toxic gain of function. |
