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验证数据展示

  • WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

    WB analysis of COLO 320 using 16818-1-AP (same clone as 16818-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

    IF Staining of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

    Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

    IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

    IHC staining of human tonsillitis using 16818-1-AP (same clone as 16818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

    IP experiment of PC-3 using 16818-1-AP (same clone as 16818-1-PBS)

    IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug. This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

    FC experiment of HepG2 using 16818-1-AP (same clone as 16818-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16818-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EED Polyclonal antibody, PBS Only

EED Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16818-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16818-1-PBS targets EED in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10358

Product name: Recombinant human EED protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-466 aa of BC068995

Sequence: MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSGRAILHSHQQCMRDPVSPNLRQHLSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 embryonic ectoderm development
别名
计算分子量 466 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC068995
基因名称 EED
Gene ID (NCBI) 8726
RRIDAB_2262065
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75530
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

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