验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
67008-1-Ig targets Endothelin 1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28334 Product name: Recombinant human EDN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-212 aa of BC009720 Sequence: MDYLLMIFSLLFVACQGAPETAVLGAELSAVGENGGEKPTPSPPWRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGNPSRERYVTHNRAHW 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | endothelin 1 |
| 别名 | EDN1, endothelin 1, ET 1 Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1 |
| 计算分子量 | 212 aa, 24 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC009720 |
| 基因名称 | Endothelin 1 |
| Gene ID (NCBI) | 1906 |
| RRID | AB_2882325 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P05305 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Endothelin 1 antibody 67008-1-Ig | Download protocol |
| WB protocol for Endothelin 1 antibody 67008-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Trace Elem Med Biol The association between endothelial function and autoimmune thyroiditis induced by iodine excess | ||
Biomed Rep Cilostazol regulates the expressions of endothelin-1 and endothelial nitric oxide synthase via activation of the p38 MAPK signaling pathway in HUVECs | ||
Hypertens Res Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation |
