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EDEM3 Polyclonal antibody

EDEM3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 27310-1-AP

Print datasheet

Synonyms

C1orf22, EDEM3

验证数据展示

  • WB analysis of 3T3-L1 using 27310-1-AP

    WB analysis of 3T3-L1 using 27310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27310-1-AP (EDEM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 27310-1-AP

    IP experiment of HEK-293 using 27310-1-AP

    IP result of anti-EDEM3 (IP:27310-1-AP, 4ug; Detection:27310-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • IHC staining of human stomach cancer using 27310-1-AP

    IHC staining of human stomach cancer using 27310-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27310-1-AP

    IHC staining of human stomach cancer using 27310-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in3T3-L1 cells, HEK-293 cell
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27310-1-AP is EDEM3 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse
Immunogen EDEM3 fusion protein Ag26123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ER degradation enhancer, mannosidase alpha-like 3
Synonyms C1orf22, EDEM3
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC016464
Gene Symbol EDEM3
Gene ID (NCBI) 80267
RRIDAB_2880839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZQ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EDEM3 antibody 27310-1-APDownload protocol
IHC protocol for EDEM3 antibody 27310-1-APDownload protocol
IP protocol for EDEM3 antibody 27310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols