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验证数据展示

  • WB analysis using 32425-1-AP

    WB analysis using 32425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32425-1-AP (EDDM3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of human testis using 32425-1-AP

    IP experiment of human testis using 32425-1-AP

    IP result of anti-EDDM3B (IP:32425-1-AP, 4ug; Detection:32425-1-AP 1:500) with human testis tissue lysate 4080 ug.

EDDM3B Polyclonal antibody

EDDM3B Polyclonal Antibody for WB, IP, ELISA
Cat No. 32425-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inhuman testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32425-1-AP targets EDDM3B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EDDM3B fusion protein Ag37630 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 12, member B (epididymal)
别名
计算分子量17 kDa
观测分子量18 kDa
GenBank蛋白编号NM_022360
基因名称 FAM12B
Gene ID (NCBI) 64184
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP56851
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDDM3B (Epididymal secretory protein E3-beta), also known as FAM12B, HE3B (Human epididymis-specific protein 3-beta), is highly enriched in the epididymis and is involved in the secretion of glycoproteins that interact with sperm membranes.

实验方案

Product Specific Protocols
WB protocol for EDDM3B antibody 32425-1-APDownload protocol
IP protocol for EDDM3B antibody 32425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols