验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32485-1-AP targets EDARADD in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38064 Product name: Recombinant human EDARADD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC128082 Sequence: MGLRTTKQMGRGTKAPGHQEDHMVKEPVEDTDPSTLSFNMSDKYPIQDTELPKAEECDTITLNCPRNSDMKNQGEENGFPDSTGDPLPEISKDNSCKENCTCSSCLLRAPTISDLLNDQDLLDVIRIKLDPCHPTVKNWRNFASKWGMSYDELCFLEQRPQSPTLEFLLRNSQRTVGQLMELCRLYHRADVEKVLRRWVDEEWPKRERGDPSRHF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | EDAR-associated death domain |
| 别名 | Ectodysplasin-A receptor-associated adapter protein, EDAR-associated death domain protein, Protein crinkled homolog |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC128082 |
| 基因名称 | EDARADD |
| Gene ID (NCBI) | 128178 |
| RRID | AB_3742630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WWZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EDARADD (EDAR-associated death domain) is an adaptor protein that mediates the interaction between the extracellular Ectodysplasin A receptor (EDAR) and intracellular signaling components, orchestrating a series of downstream events that regulate key biological processes such as cell proliferation, differentiation, and apoptosis. EDARADD is a 25 KDa death domain-containing adaptor protein. EDARADD was shown to interact with TRAF1, -2 and -3 as well as with TAB2 (TAK1-binding protein 2) and is therefore likely to be responsible for coupling receptor activation with engagement of the NF-κB pathway (PMID: 23845861).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EDARADD antibody 32485-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
