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  • Featured Product
  • KD/KO Validated

ECOP Polyclonal antibody

ECOP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12611-1-AP

Print datasheet

Synonyms

ECOP, GASP, VOPP1

验证数据展示

  • WB analysis of Transfected HEK-293 using 12611-1-AP

    WB analysis of Transfected HEK-293 using 12611-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12611-1-AP (ECOP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 12611-1-AP

    IHC staining of human kidney using 12611-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12611-1-AP (ECOP Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inTransfected HEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12611-1-AP targets ECOP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ECOP fusion protein Ag3225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EGFR-coamplified and overexpressed protein
Synonyms ECOP, GASP, VOPP1
Calculated Molecular Weight 172 aa, 19 kDa
GenBank Accession NumberBC016650
Gene Symbol ECOP
Gene ID (NCBI) 81552
RRIDAB_2877866
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AW1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECOP, also named as VOPP1 and GASP, has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. It interacts directly with cytoplasmic mediators of the NF kappa B pathway. ECOP has three isoforms with MW 18-19 kDa.

实验方案

Product Specific Protocols
WB protocol for ECOP antibody 12611-1-APDownload protocol
IHC protocol for ECOP antibody 12611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF,IP

BMC Biol

VOPP1 promotes breast tumorigenesis by interacting with the tumor suppressor WWOX.

Authors - Florian Bonin
  • KD Validated
View Article
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
View Article