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验证数据展示

  • WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

    WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB analysis of MCF-7 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB result of ECHS1 antibody (66117-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

    IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ECHS1 Monoclonal antibody, PBS Only

ECHS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66117-1-PBS

产品说明书

CloneNo. 2B9D5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

产品信息

66117-1-PBS targets ECHS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16775

Product name: Recombinant human ECHS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-290 aa of BC008906

Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
别名 mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC008906
基因名称 ECHS1
Gene ID (NCBI) 1892
RRIDAB_2881516
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30084
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

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