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验证数据展示

  • WB analysis of PC-12 using 27732-1-AP

    WB analysis of PC-12 using 27732-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27732-1-AP (ECHDC1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of mouse kidney using 27732-1-AP

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHDC1 antibody included
  • IHC staining of mouse kidney using 27732-1-AP

    IHC staining of mouse kidney using 27732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27732-1-AP (ECHDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECHDC1 antibody included

ECHDC1 Polyclonal antibody

ECHDC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27732-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

ECHDC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, A549 cells
Positive IHC detected inmouse kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECHDC1 Ready-To-Use IHC Kit. ECHDC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27732-1-AP targets ECHDC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ECHDC1 fusion protein Ag26905 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enoyl Coenzyme A hydratase domain containing 1
别名 ECHDC1
计算分子量 307 aa, 34 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC003549
基因名称 ECHDC1
Gene ID (NCBI) 55862
RRIDAB_2880956
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NTX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECHDC1 antibody 27732-1-APDownload protocol
IHC protocol for ECHDC1 antibody 27732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols